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KMID : 0380020100250040357
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 4 p.357 ~ p.362
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Enhancement of Saccharification Yield of Ulva pertusa kjellman for Ethanol Production through High Temperature Liquefaction Process
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Han Jae-Gun
Oh Sung-Ho
Choi Woon-Yong
Kwon Jung-Woong
Seo Hyeon-Beom
Jung Kyung-Hwan
Kang Do-Hyung
Lee Hyeon-Yong
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Abstract
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Green alga, Ulva pertusa kjelmann has been known to be one of the largest pollutants in Korea. Therefore, the efficient pretreatment processes have been required to improve the yields of fermentable sugar. The optimal pretreatment conditions were determined to be 195℃ for 15 min. The sugar yield of glucose and xylose were estimated as 20.5%, and 5.0% respectively, based on theoretical yields. However solid residues were estimated enzymatic digestibility of 90-95% with cellulase loading of 15 FPU/g glucan. This process was proved to generate the low concentration of Hydroxy-Methyl-Furfural (51 ppm), which resulted in ethanol production with 95% of the maximum conversion yield from glucose in the culture of Saccharomyces cerevisiae (ATCC, 24858). This study showed that Ulva pertusa kjellmann can be used as a bioetahnol resource using the high temperature liquefaction process.
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KEYWORD
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Ulva pertusa kjelmann, pretreatment, fermentable sugar, HMF, ethanol production
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